BJO

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS REGISTER


[Advanced]
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this link to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Add article to my folders
Right arrow Download to citation manager
Right arrowRequest Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Woo, H-M
Right arrow Articles by Kim, J H
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Woo, H-M
Right arrow Articles by Kim, J H
Br J Ophthalmol 2001;85:345-349 ( March )

Effects of amniotic membrane on epithelial wound healing and stromal remodelling after excimer laser keratectomy in rabbit cornea

H-M Woob, M S Kima, O-K Kweonb, D-Y Kimb, T-C Namb, J H Kima

a Department of Ophthalmology, Kangnam St Mary's Hospital, The Catholic University of Korea, College of Medicine, Seoul, Korea, b College of Veterinary Medicine, Seoul National University, Suwon, Korea

Correspondence to: Dr M S Kim mskim{at}cmc.cuk.ac.kr

Accepted for publication 10 July 2000

AIMS---To investigate if the amniotic membrane (AM) promotes epithelial migration while inhibiting stromal remodelling associated with corneal haze after excimer laser keratectomy.
METHODS---A wound 150 µm in depth and 6.0 mm in diameter was produced in 40 rabbits using an excimer laser. One eye was randomly chosen to be covered by the AM while the other eye served as a control. Epithelial wound healing was evaluated, together with any morphological changes of the anterior stroma connected with corneal haze. These morphological changes were histopathologically analysed using dichlortriazinyl aminofluorescein (DTAF), Masson trichrome staining, and an image analyser.
RESULTS---The AM group had a short latent phase followed by fast epithelial healing (p<0.001) during the early wound healing period and a significant decrease in the inflammatory response, together with a smaller change in the number of keratocytes than the control group. The mean thickness of the regenerated stroma was significantly thinner in the AM group than in the control group at 8 weeks (p<0.0001). The AM group had a more regular architecture of regenerated stromal lamella at 8 weeks and significantly less haze after 4 weeks than the control group (p<0.05).
CONCLUSION---Use of the AM as a dressing on a corneal wound created by excimer laser surgery, in which severe haze is expected, may induce rapid epithelial healing with less inflammatory response. The AM may inhibit the irregular synthesis of stromal collagen that is associated with corneal haze.

© 2001 by British Journal of Ophthalmology


This article has been cited by other articles:


Home page
 Br. J. Ophthalmol.Home page
M Nubile, H S Dua, T E.-M Lanzini, P Carpineto, M Ciancaglini, L Toto, and L Mastropasqua
Amniotic membrane transplantation for the management of corneal epithelial defects: an in vivo confocal microscopic study
Br. J. Ophthalmol., January 1, 2008; 92(1): 54 - 60.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
S.-E. Ti, D. Anderson, A. Touhami, C. Kim, and S. C. G. Tseng
Factors Affecting Outcome Following Transplantation of Ex vivo Expanded Limbal Epithelium on Amniotic Membrane for Total Limbal Deficiency in Rabbits
Invest. Ophthalmol. Vis. Sci., August 1, 2002; 43(8): 2584 - 2592.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS REGISTER
Terms and conditions relating to subscriptions purchased online  ¦  Website terms and conditions  ¦  Privacy policy
Copyright © 2001 by the BMJ Publishing Group Ltd.