Br J Ophthalmol 2000;84:212-216
( February )
Apoptosis and apoptosis related gene expression in normal
conjunctiva and pterygium
Donald T H Tana, Wen Ying Tangb, Yan Ping Liua, Hak-Su Gohb, Duncan R Smithb
a Singapore National
Eye Centre, 11 Third Hospital Avenue, Singapore 168751, b Molecular Biology
Laboratory, Tan Tock Seng Hospital, Moulmein Road, Singapore 308433
Correspondence to: Dr Duncan R Smith
Accepted for publication 19 November 1999
BACKGROUND Pterygium
is a relatively common eye disease in the tropics whose aetiology and
pathogenesis remain uncertain. As such, interest has focused on
understanding the underlying mechanism of pterygia development.
METHODS15 specimens
of pterygia from 15 eyes were examined, together with normal
conjunctival tissue from the same eyes for the pattern of gene
expression of genes associated with the induction or repression of
apoptosis (p53, bcl-2, and bax). In addition, the samples directly for
apoptotic cells were examined by the terminal deoxynucleotide transferase (TdT) mediated nick end labelling (TUNEL) methodology.
RESULTSIn pterygia
specimens apoptotic cells were found mainly confined to the basal layer
of cells of the epithelial layer, situated immediately adjacent to the
fibrovascular support layer. These cells were shown to express
significant levels of p53 and bax, as well as the apoptosis inhibiting
protein bcl-2. In contrast, normal conjunctival specimens displayed no
bcl-2 expression and apoptotic cells were seen throughout the entire
width of the epithelial layer, coupled with high levels of bax expression.
CONCLUSIONThese
results support a model whereby pterygia development is a result of
disruption of the normal process of apoptosis occurring in the conjunctiva.
© 2000 by British Journal of Ophthalmology
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