Br J Ophthalmol 1999;83:723-727 ( June )
PAX6 expression in the developing human eye
Sachiko Nishinaa, Shinichi Kohsakab, Yuki Yamaguchic, Hiroshi Handac, Atsushi Kawakamid, Hajime Fujisawad, Noriyuki Azumaa
a Department of
Ophthalmology, National Children's Hospital, Tokyo, Japan, b Department of
Neurochemistry, National Institute of Neuroscience, Tokyo, Japan, c Department of Biomolecular
Engineering, Faculty of Bioscience and Biotechnology, Tokyo Institute
of Technology, Kanagawa, Japan, d Division
of Biological Science, Nagoya University Graduate School of Science,
Nagoya, Japan
Correspondence to: Noriyuki Azuma, MD, Department of
Ophthalmology, National Children's Hospital, 3-35-31 Taishido,
Setagaya-ku, Tokyo, 154-8509, Japan.
Accepted for publication 11 January 1999
AIMS To investigate
the changes in PAX6 expression in the developing human eye.
METHODSSix developing
human eyes from 6 to 22 weeks' gestation were evaluated. Frozen
sections were immunohistochemically stained with monoclonal antibody to
chick Pax6 (amino acids 1-223). To verify antibody specificity,
western blot analysis was carried out using cell lysates from P19 cells
transfected with the human PAX6 gene.
RESULTSWestern blot
analysis demonstrated that the antibody reacted to human PAX6 protein.
Positive immunostainings for PAX6 were seen in the surface
ectoderm, lens vesicle, inner and outer layers of the optic cup, and
optic stalk at 6 weeks, and in the corneal epithelia and conjunctiva,
lens, and non-pigmented ciliary epithelia from 8 to 22 weeks. In the
retina, positive cells were seen in the entire retina from 8 to 10 weeks, and were restricted to the ganglion cell layer and the inner and
outer portions of the inner nuclear layer after 21 weeks.
CONCLUSIONSPAX6
is expressed on the surface and neuroectoderms at an early stage, then
in the differentiating cells in the cornea, lens, ciliary body, and
retina through development. PAX6 may play a role in determining cell
fate in the morphogenesis of various human ocular tissue.
© 1999 by British Journal of Ophthalmology
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