Br J Ophthalmol 1999;83:598-604 ( May )
Dendritic cells and macrophages in the uveal tract of the normal
mouse eye
Paul G McMenamin
Department of
Anatomy and Human Biology, University of Western Australia, Nedlands,
Perth 6907, Western Australia
Accepted for publication 2 December 1998
BACKGROUND/AIMS Dendritic
cells (DC) and macrophages are components of the immune cell
populations in the uveal tract whose density, distribution, turnover,
and function may play a role in the maintenance of immunological homeostasis in the eye. Little is known of these cells in the mouse eye
despite this being the predominant experimental model in many studies
of ocular immune responses and immunoinflammatory mediated eye
diseases. The aim of the present study was to obtain further
immunophenotypic data on resident tissue macrophages and DC populations
in the mouse uveal tract.
METHODSPieces of
iris, ciliary body, and choroid dissected from perfusion fixed BALB/c
mice were incubated whole in a variety of anti-macrophage and DC
monoclonal antibodies (mAbs). Labelled cells were visualised using
either single or double immunoperoxidase techniques.
RESULTSQuantitative
analysis and double immunolabelling revealed that 80% of
F4/80+ cells (a mAb that recognises both DC and
macrophages) in the iris are macrophages (SER4+). The iris
contained a network of Ia+ cells (412 (SD 130)
cells/mm2) of which two thirds appear to be DC. A similar
pattern was observed in the ciliary body and choroid. Only a few DC in
the uveal tract were very weakly reactive for mAbs which recognise
B7-1 (CD80), B7-2 (CD86),  2 integrin (mAb N418), and
multivesicular bodies associated with antigen presentation (mAb M342).
CONCLUSIONSThe
present study reveals that the mouse uveal tract, like the rat,
contains rich networks of DC and resident tissue macrophages. The
networks of resident tissue macrophages in the mouse uveal tract
closely resemble similar networks in non-ocular tissues. The phenotype
of uveal tract DC suggests they are in the "immature" phase of
their life cycle, similar to Langerhans cells of the skin, thus
implying their role in situ within the eye is antigen capture and not
antigen presentation.
© 1999 by British Journal of Ophthalmology
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