Br J Ophthalmol 1999;83:231-235 ( February )
Histochemical localisation of mitochondrial enzyme activity in
human optic nerve and retina
Richard M Andrews,a
Philip G Griffiths,a
Margaret A Johnson,b
Douglass M Turnbullb
a Department of
Ophthalmology, University of Newcastle upon Tyne, b Department of Neurology,
University of Newcastle upon Tyne
Correspondence to: Mr Richard Andrews, Department of Neurology, The Medical
School, Framlington Place, Newcastle upon Tyne, NE2 4HH.
Accepted for publication 1 September 1998
AIMS To demonstrate
the quantitative distribution of mitochondrial enzymes within the human
optic nerve and retina in relation to the pathogenesis of ophthalmic disease.
METHODSEnucleations
were performed at the time of multiple organ donation and the optic
nerve and peripapillary retina immediately excised en bloc and frozen.
Reactivities of the mitochondrial enzymes cytochrome
c oxidase and succinate dehydrogenase were demonstrated in serial cryostat sections using specific histochemical assays.
RESULTSIn the optic
nerve the unmyelinated prelaminar and laminar regions were rich in both
cytochrome c oxidase and succinate
dehydrogenase. Myelination of fibres as they exited the lamina cribrosa
was associated with an abrupt reduction in enzyme activity. Within the
retina, high levels of enzyme activity were found localised within the retinal ganglion cells and nerve fibre layer, the outer plexiform layer, inner segments of photoreceptors, and the retinal pigment epithelium.
CONCLUSIONSMitochondrial
enzyme activity is preserved in human optic nerve and retina retrieved
at the time of multiple organ donation. The distribution of enzyme
activity within the eye has implications for the understanding of the
pattern of ophthalmic involvement seen in mitochondrial diseases and
the site of ganglion cell dysfunction in those patients with optic
nerve involvement.
Keywords:
mitochondria;
optic nerve;
retina
© 1999 by British Journal of Ophthalmology
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